Stony Brook researchers discover a method to change emotionally charged memory patterns.
Imagine if memory could be tuned in such a way where good memories are enhanced for those suffering from dementia or bad memories are wiped away for individuals with post-traumatic stress disorder. A Stony Brook University research team has taken a step toward the possibility of tuning the strength of memory by manipulating one of the brain’s natural mechanisms for signaling involved in memory, a neurotransmitter called acetylcholine. Their findings are published in the journal Neuron.
Brain mechanisms underlying memory are not well understood, but most scientists believe that the region of the brain most involved in emotional memory is the amygdala. Acetylcholine is delivered to the amygdala by cholinergic neurons that reside in the base of the brain. These same neurons appear to be affected early in cognitive decline. Previous research has suggested that cholinergic input to the amygdala appears to strengthen emotional memories.
“Memories of emotionally charged experiences are particularly strong, whether positive or negative experiences, and the goal of our research is to determine the mechanisms underlying the strengthening of memory,” said Lorna Role, PhD, Professor and Chair of the Department of Neurobiology and Behavior and Co-Director of the Neurosciences Institute at Stony Brook Medicine.
In the paper, titled “Cholinergic Signaling Controls Conditioned Fear Behaviors and Enhances Plasticity of Cortical-Amygdala Circuits,” Dr. Role and colleagues used a fear-based memory model in mice to test the underlying mechanism of memory because fear is a strong and emotionally charged experience.
The team used optogenetics, a newer research method using light to control cells in living tissue, to stimulate specific populations of cholinergic neurons during the experiments.
Two of the team’s findings stand out. First, when they increased acetylcholine release in the amygdala during the formation of a traumatic memory, it greatly strengthened memory making the memory last more than twice as long as normal. Then, when they decreased acetylcholine signaling in the amygdala during a traumatic experience, one that normally produces a fear response, they could actually wipe out memory.
“This second finding was particularly surprising, as we essentially created fearless mice by manipulating acetylcholine circuits in the brain,” explained Dr. Role. “The findings provide the basis for research examining novel approaches to reverse post-traumatic stress disorder.”
The challenge of continued research is that cholinergic neurons remain difficult to study because they are intermingled with other types of neurons and are few in number compared to other types of neurons in the brain.
Because acetylcholine is a natural signaling mechanism and seemingly essential for memory, additional research will center on non-pharmacologic ways to manipulate or fine-tune memory.
“The long-term goal of our research is that we would like to find ways – potentially independent of drug administration – to enhance or diminish the strength of specific memories, the good ones, and diminish the bad ones,” summarized Dr. Role.
The research involves faculty and students from the Stony Brook University Departments of Neurobiology and Behavior, and Pharmacological Sciences, as well as the CNS Disorders Center, the Neurosciences Institute, and the Program in Neurosciences. Co-authors include Li Jiang, Srikanya Kunda, James D. Lederman, Gretchen Y. Lopez-Hernandez, Elizabeth C. Ballinger, Shaohua Wang, and David A. Talmage.
Source: Gregory Filiano – Stony Brook University
Image Source: The image is in the public domain.
Original Research: Abstract for “Cholinergic Signaling Controls Conditioned Fear Behaviors and Enhances Plasticity of Cortical-Amygdala Circuits” by Li Jiang, Srikanya Kundu, James D. Lederman, Gretchen Y. López-Hernández, Elizabeth C. Ballinger, Shaohua Wang, David A. Talmage, Lorna W. Role in Neuron. Published online May 5 2016 doi:10.1016/j.neuron.2016.04.028
Cholinergic Signaling Controls Conditioned Fear Behaviors and Enhances Plasticity of Cortical-Amygdala Circuits
•Photostimulation of ACh in BLA during cue-fear training makes memory more durable
•Stimulating ACh input to BLA in vivo and ex vivo increases neuronal excitability
•Stimulating ACh input to BLA can elicit LTP
•All of the above effects are dependent on acetylcholine receptors (AChRs)
We examined the contribution of endogenous cholinergic signaling to the acquisition and extinction of fear- related memory by optogenetic regulation of cholinergic input to the basal lateral amygdala (BLA). Stimulation of cholinergic terminal fields within the BLA in awake-behaving mice during training in a cued fear-conditioning paradigm slowed the extinction of learned fear as assayed by multi-day retention of extinction learning. Inhibition of cholinergic activity during training reduced the acquisition of learned fear behaviors. Circuit mechanisms underlying the behavioral effects of cholinergic signaling in the BLA were assessed by in vivo and ex vivo electrophysiological recording. Photostimulation of endogenous cholinergic input (1) enhances firing of putative BLA principal neurons through activation of acetylcholine receptors (AChRs), (2) enhances glutamatergic synaptic transmission in the BLA, and (3) induces LTP of cortical-amygdala circuits. These studies support an essential role of cholinergic modulation of BLA circuits in the inscription and retention of fear memories.
“Cholinergic Signaling Controls Conditioned Fear Behaviors and Enhances Plasticity of Cortical-Amygdala Circuits” by Li Jiang, Srikanya Kundu, James D. Lederman, Gretchen Y. López-Hernández, Elizabeth C. Ballinger, Shaohua Wang, David A. Talmage, Lorna W. Role in Neuron. Published online May 5 2016 doi:10.1016/j.neuron.2016.04.028